Soto M, Zorzano A, Olivares J and Toro N 1992 Sequence of IS Rm4 from Rhizobium meliloti strain GR4. Sarno R and Stringi L 1981 Sulla ( Hedysarum coronarium L.) in coltivazioni Erbacee. Sanger F, Nicklen S and Coulson A R 1977 DNA sequencing with chain terminating inhibitors. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, USA. Sambrook J, Fritsch E F and Maniatis T 1989 Molecular Cloning. Ruvkan G B, Long S R, Meade H M, van den Bos R C and Ausubel F M 1982 ISRml: a Rhizobium meliloti insertion sequence that transposes preferentially into nitrogen fixation genes. ( Leucaena diversifolia) and distribution of homologous sequences identifying cross-inoculation group relationships.
Rice J D, Somasegaran P, MacGlashnan K and Bohlool B B 1994 Isolation of insertion sequence IS RLdTAL1145–1 from a Rhizobium sp. Ollero F J, Valverde M A, Espuny M R and Bellogin R A 1993 In vivo formation of R-prime plasmids harbouring nod genes of Rhizobium “hedysari”. Ollero F J, Espuny M R and Bellogin R A 1989 Mobilization of symbiotic plasmid from a strain of Rhizobium sp. Lupi F, Casella S, Tofanin A and Squartini A 1988 Introduction of Rhizobium “hedysari” in alkaline clay-loam soil by different inoculation techniques. Kosier B, Puhler A, and Simon R 1993 Monitoring the diversity of Rhizobium meliloti field and microcosm isolates with a novel rapid genotyping method using insertion elements. Higgins DG and Sharp PM 1988 Clustal: a package for performing multiple seqence alignment on a microcomputer. trifolii symbiotic plasmid pRtr5a to a strain of Rhizobium sp. Gene 68, 119–138.Įspuny M R, Ollero F J, Bellogin R A, Ruiz-Sainz J E and Perez-Silva J 1987 Transfer of the Rhizobium leguminosarum biovar. 169, 1403–1409.Įlhai J and Wolk C P 1988 A versatile class of positive selection vectors based on the nonviability of palindrome-containing plasmids that allows cloning into long polylinkers. Publ., Oxford, UK.ĭusha I, Kovalenko S, Banfalvi Z and Kondorosi A 1987 Rhizobium meliloti insertion element ISRm2 and its use for identification of the fixX gene. 22, 37–45.ĭazzo F 1984 Leguminous root nodules. p 294.Ĭasella S, Gault R R, Reynolds K C, Dyson J R and Brockwell J 1984 Nodulation studies on legumes exotic to Australia: Hedysarum coronarium. In VII Congreso Nacional de Microbiologia, Cadiz (Abstr. 215, 403–410.Ĭabrera E, Villa A and Ruiz-Argüeso T 1979 Diversidad y caraterizaciòn de la flora nativa de rizobios que nodulan en zulla ( Hedysarum coronarium). The significance of this IS element in rhizobia and its possible use as a probe for taxonomic and phylogenetic studies of Rhizobiaceae is addressed.Īltschul S F, Gish W, Myers E W and Lipman D J 1990 Basic local alignment search tool. The six copies in strain IS 123 appear clustered specifically within the pSym nod region. Hybridization studies revealed that several strains of Rhizobium ‘hedysari’ carry this element in various copy number. This 0.8 kb novel element carries two 17-bp flanking inverted repeats and an open reading frame showing homology with a transposase from Staphylococcus aureus. This DNA region also contained at least six copies of a novel insertion sequence-like structure, some of which appeared to have suffered deletions. Cloning and sequencing of DNA from a symbiotic large plasmid in Rhizobium ‘hedysari’ strain IS 123 required for its nodulation of the mediterranean legume crop Hedysarum coronarium (sulla) and complementation studies of nod - mutant derivatives led to the characterization of a 30-kb region containing common and host-specific nod genes.
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